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KMID : 0903519840270020073
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1984 Volume.27 No. 2 p.73 ~ p.78
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Studies on the Exo - maltotetraohydrolase of Pseudomonas stutzeri IAM 12097 - Part Ⅰ. Purification of Exo - maltotetraohydrolase -
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Abstract
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The optimum culture time and initial pH, for the production of exo-maltotetraohydrolase from Pseudomonas stutzeri IAM 12097, in the trypticase medium were 36 hrs and pH 6.3, respectively. Exo-maltotetraohydrolase was purified by (NH₄)₂SO₄ and two times of column chromatography on DEAE-cellulose. Specific activity of the purified enzyme was 108.6U/㎎-protein and yield of the enzyme activity was 9.4%. The purified enzyme shoved a single band on polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis.
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